Strep-tag technology for protein purification and detection is covered by US patent 5,506,121, UK patent 2272698 and French patent 93 13 066 and Strep-Tactin is covered by US patent 6,103,493. Hoffmann-La Roche owns patents and patent applications pertaining to the application of Ni-NTA resin (Patent series: RAN 4100/6 3: USP 4.877.830, USP

Strep-Tactin multimers conjugated with magnetic microbeads. €205.00*. Prices excl. VAT plus shipping costs. Content. 750 l. Description. Strep-Tactin Magnetic Microbeads are microspheres covalently linked to Strep-Tactin . They are suitable for labeling antige…. More

The binding capacity of the resin was several-fold higher for the tag located at the N-terminus of the protein as opposed to the C-terminus- or middle of the fusion. The variation in the length of the linker between the double repeats of the Strep-tag from 6 to 12

Nov 27, 2020 [5, 13] Whole proteins (like the HaloTag) or small peptide sequences (e.g., His-tag, Strep-Tag) can be used as affinity tags. [14-16] Using whole proteins as affinity tags often leads to a compromised activity of the fused partner, due to their size. Thus, the usage of smaller affinity tags seems reasonable

Strep-tags are short peptides with high binding selectivity for Strep-Tactin, an engineered streptavidin. The binding affinity of e.g. Strep-tag II to Strep-Tactin (Kd = 1 M) is nearly 100 times higher than to streptavidin. Strep-tags may be fused to recombinant proteins which allows efficient one-step purification of such fusion proteins on immobilized Strep– Tactin under […]

Recombinant proteins that carry two small affinity tags (the 6xHis tag and Strep-tag II) are efficiently expressed in E. coli, insect, or mammalian cells using pQE-TriSystem His Strep vectors. After cell lysis and clearing of the lysate, proteins are initially purified using an immobilized-metal affinity chromatography procedure that is based on the proven 6xHis-tag-Ni-NTA interaction

Strep Tag (N-term) Yes. pSTREPHIS1525. BMEG15. 6xStrepII N-terminal tag (cleavable), 6xHis C-terminal tag. Yes. pC-His1622. BMEG20. 6xHis C-terminal tag : pC-Strep1622. BMEG21. 6xStrepII C-terminal tag : pN-His-TEV1622. BMEG22. 6xHis N-terminal tag, cleavable . pN-Strep-TEV1622. BMEG23. 6xHis StrepII N-terminal tag, cleavable . pN-StrepXa1622

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Western Blotting (protein blotting or immunoblotting), is an important technique to detect specific proteins in a sample of tissue homogenate or extract. When performing a Western Blot, it's a wise idea to follow your procedure step by step. To perform a Western Blot successfully, every single step should not

For subsequent purification of the protein of interest, two vectors are offered encoding for 8xHis- or Strep tag II: pHT2133 (PBS008) - pHT2133 vector with C-terminal encoded 8xHis tag; pHT2134 (PBS009) - pHT2134 vector with C-terminal encoded Strep tag; A control vector is also available

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4 Streptamer Manual ΠAntigen-specific staining and functional isolation of T-cells 2 The Streptamerfi Principle Strep-tags , Strep-Tactin and Streptamers Strep-tags are short peptides with high binding selectivity for Strep-Tactin, an engineered streptavidin. The binding affinity of e.g. Strep-tag II to Strep-Tactin (Kd = 1 M) is nearly 100 times higher than to streptavidin

The Strep-tag II binds specifically to the engineered streptavidins, Strep-Tactin and Strep-Tactin XT, by occupying the binding pocket of the natural ligand biotin. Hence, the interaction is easily reversible by excessive addition of the competitor. Two affinity tags – two streptavidin derivatives: The Strep-tag

The small Strep-tag II (WSHPQFEK) can be used to purify active, native conformation proteins to high purity on Strep-Tactin resins.In addition, it can be used in combination with the His tag in a standardized two-step purification procedure. Strep-Tactin is available coupled to magnetic beads for small-scale purification procedures (up to 100 μg) or Superflow chromatography resin for larger

The Strep-tag is a nine-amino-acid peptide that was developed as an artificial ligand for streptavidin. Further refinements to these molecules yielded the eight-amino-acid peptide Strep-tag II and a mutant form of streptavidin called Strep-Tactin capable of moderate-affinity binding (KD ~ 1 M)

MagStrep “type3” XT beads are magnetic beads consisting of a 6% agarose coated with the streptavidin variant Strep-Tactin XT and can be used for Strep-tag II and Twin-Strep-tag fusion proteins. Due to the magnetic core no centrifugation step is required since the beads can be easily and swiftly separated with a magnetic separator, reducing the risk of disturbing the pellet or loss of

The streptavidin-binding tag (Strep-tag) is a small affinity protein that is used as a sorbent. Because the affinity of biotin for streptavidin is higher than that of any of the streptavidin-binding peptides, Strep-tagged fusion proteins can be efficiently eluted by free biotin. Strep-tag is infrequently used as it is restricted by its location

Strep Tag (N-term) Yes. pSTREPHIS1525. BMEG15. 6xStrepII N-terminal tag (cleavable), 6xHis C-terminal tag. Yes. pC-His1622. BMEG20. 6xHis C-terminal tag : pC-Strep1622. BMEG21. 6xStrepII C-terminal tag : pN-His-TEV1622. BMEG22. 6xHis N-terminal tag, cleavable . pN-Strep-TEV1622. BMEG23. 6xHis StrepII N-terminal tag, cleavable . pN-StrepXa1622

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Immunoprecipitate or affinity purify antibodies (IgG) using Protein A/G coated magnetic particles for manual or robotic magnetic separation. Magnetic beads for recombinant c-Myc-tagged protein purification. For use with GeneCatcher™ gDNA 0.3 - 1.0mL

Protein tag refers to the sub-domain or the peptide sequence of a fusion protein (see What is Fusion Protein) that is linked to the interest protein.There are many kinds of protein tags which are widely used in recombinant protein production.Protein tags are useful and convenient tool for improving solubility of recombinant proteins, streamlining protein purification, and allowing an easy way

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