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Concentration shaking table s6

Sep 08, 2015 S6 Table: In vivo assembly of Clostridium ... (New England Biolabs; CutSmart buffer was added to final concentration of 1 for all digests) for ~2 hours at 37 C. Nucleospin Gel and PCR Clean-Up kit (Macherey-Nagel) was used for PCR product purification. ... and the cells were allowed to recover at 37 C with shaking at 250 rpm for one hour

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